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KMID : 0603820130190010009
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Journal of Experimental & Biomedical Science
2013 Volume.19 No. 1 p.9 ~ p.16
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Characterization of Matrix Metalloproteinase Expression in Triglyceride Treated THP-1 Macrophages
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Cho Yoon-Jung
Lim Jae-Won
Lee Dong-Hyun
Jung Byung-Chul
Lee Min-Ho
Jung Dong-ju
Kim Yoon-Suk
Kim Tae-Ue
Rhee Ki-Jong
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Abstract
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Elevated blood triglyceride (TG) levels correlate with development of atherosclerosis suggesting that TG may promote the development of this disease. During atherosclerosis, TG is taken up by tissue macrophages which result in dramatic changes in various secreted factors. One such factor is the family of matrix metalloproteases (MMP) which are involved in tissue remodeling during both physiological and pathological processes. In this study, we examined the MMP expression profile in PMA-differentiated THP-1 macrophages treated with TG. We found that TG-treated THP-1 macrophages showed decreased expression of MMP-3, MMP-7, MMP-8 and MMP-9 in a time- and dose-dependent manner. In contrast, expression of MMP-1, MMP-2, and MMP-10 remained relatively unchanged after TG treatment. In addition, we found that expression of select MMPs was affected by various inhibitors of signaling pathways. In particular, expression of MMP-3 was slightly recovered by cRAF and PLC signaling pathway inhibitors. These data suggests a possible role of MMPs in macrophages during TG-induced atherosclerosis.
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KEYWORD
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Triglyceride, Matrix metalloprotease, THP-1 macrophages, Atherosclerosis
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